Molecular Biology calculators
Tools for working with DNA and RNA: converting absorbance to concentration, diluting a stock to a working concentration, checking the GC content and reverse complement of a sequence, estimating a primer's melting temperature, scaling a PCR master mix, and calculating relative expression from qPCR data.
The sequence tools accept pasted FASTA records and ignore headers, spaces and numbering. The primer Tm calculator uses published nearest-neighbour thermodynamics and states which effects it does not model.
DNA and RNA Concentration Calculator (A260)Nucleic acid concentration and purity from absorbanceOpen calculatorDNA Dilution CalculatorDilute a DNA or RNA stock to a working concentrationOpen calculatorGC Content CalculatorGC percentage and base composition of a sequenceOpen calculatorReverse Complement ToolReverse, complement or reverse-complement a sequenceOpen calculatorPrimer Tm Calculator (Four Methods)Primer melting temperature by four accepted methods, side by sideOpen calculatorDNA and RNA Molecular Weight CalculatorExact molecular weight of an oligo or sequence, ss or dsOpen calculatorDNA and RNA Molarity CalculatorConvert ng/µL to nM, pmol per µg and molecules per µLOpen calculatorDNA Copy Number CalculatorNumber of copies in a mass of plasmid, amplicon or genomeOpen calculatorFASTA Sequence AnalyzerLength, GC, base counts and CpG ratio for one or many sequencesOpen calculatorDNA to RNA Converter (Transcription)Transcribe DNA to mRNA, back to DNA, or to cDNAOpen calculatorDNA Translation Tool (DNA to Protein)Translate in any of six reading frames with a chosen genetic codeOpen calculatorCodon Usage CalculatorCodon counts, frequency per thousand, RSCU and GC3Open calculatorORF FinderOpen reading frames in all six frames, ATG to stopOpen calculatorIUPAC Ambiguity Code ToolDecode ambiguity codes and count the sequences they representOpen calculatorSequence Comparison and Alignment ToolPercent identity by global alignment or position by positionOpen calculatorGC Content Profile (Sliding Window)GC percentage along a sequence in a moving windowOpen calculatorOligo Resuspension and Dilution CalculatorBuffer volume for a stock, and dilution to a working concentrationOpen calculatorOligo Concentration Calculator (A260)Oligo concentration from absorbance, and µM ⇄ ng/µLOpen calculatorOligo and Primer AnalyzerLength, GC, Tm, weight and a design-guideline check for a primerOpen calculatorPrimer Hairpin and Dimer CheckerSelf-complementary stems and primer-dimer runs by sequence matchingOpen calculatorDegenerate Primer GeneratorBack-translate a peptide into a degenerate IUPAC primerOpen calculatorPrimer Pair AnalyzerTm match, GC, dimers and product size for a forward-reverse pairOpen calculator
Related equipment
Service documentation, failure modes and parts for the instruments these calculations are used with.