Cell Culture calculators
The calculations that come up at every passage: turning a hemocytometer count into cells per mL, measuring viability, diluting a suspension, seeding wells or flasks at a chosen density, splitting a culture, and tracking growth as doubling time and population doubling level.
Counts are reported with their sampling uncertainty, because a count of fifty cells and a count of five hundred are not equally trustworthy. Estimates that depend on a property of your own cell line, such as confluence, are labelled as estimates.
Trypan Blue Cell Count CalculatorViable cell concentration and viability from a trypan blue countOpen calculatorMOI Calculator (Multiplicity of Infection)Virus volume for a target MOI, or the MOI from a volume, with Poisson sharesOpen calculatorCulture Medium Supplement CalculatorVolumes of serum and supplements for a bottle of complete mediumOpen calculatorCell Freezing Calculator (Cryopreservation)Cells, freezing medium and DMSO for a batch of vialsOpen calculatorHemocytometer Cell Counting CalculatorCells per mL from a hemocytometer countOpen calculatorTotal Cell Count CalculatorTotal and viable cells in a suspensionOpen calculatorCell Viability Calculator (Trypan Blue)Percent viability from live and dead countsOpen calculatorCell Dilution CalculatorDilute a cell suspension to a target densityOpen calculatorCell Seeding CalculatorSuspension and medium for a plate of wellsOpen calculatorCells per cm² Seeding Density CalculatorCells needed for a vessel at a given seeding densityOpen calculatorSplit Ratio CalculatorSuspension and medium per vessel when passagingOpen calculatorCell Doubling Time CalculatorDoubling time from two cell countsOpen calculatorPopulation Doubling Level CalculatorPopulation doublings and cumulative PDL per passageOpen calculatorCell Confluence CalculatorEstimated confluence and time to reach a targetOpen calculator
Related equipment
Service documentation, failure modes and parts for the instruments these calculations are used with.