Pharmacia LKB LKB Biochrom ULTROSPEC II
Pharmacia LKB · Model LKB Biochrom ULTROSPEC II
Spectrometer Ease of operation is achieved by using a microprocessor to perform most of the repetitive talks usually requiring prolonged operator action. The instrument is capable of performing absorbance, concentration and transmittance measurements on up to five samples plus on reference at wavelengths in the 200 nm to 900 nm range. Ideal for biological samples when using standard 10 mm cuvettes. Pharmacia LKB Biochrom ULTROSPEC II is designed as a simple to use instruments capable of producing rapid measurement of light absorption or transmission. The LKB Biochrom Ultrospec II UV/Vis Spectrophotometer is designed to produce rapid measurements of light absorption or transmission in both the ultra-violet and visible spectra. Capable of performing the absorbance, concentration and transmittance measurements on up to five samples plus one reference, the Ultrospec 4050 features wavelengths ranges from 200nm to 900nm.
Typical Failure Modes
Compiled for this model from its own documentation and established engineering knowledge for this equipment class - not from a verified service record. Confirm against the manufacturer's service manual.
Low lamp energy / high photometric noise
HighSymptoms: Low-energy warning, noisy baseline, poor signal-to-noise, worse in the UV.
Root cause: Deuterium (UV) or tungsten (visible) lamp at end of life.
Baseline / photometric drift
MediumSymptoms: Baseline will not flatten, absorbance offset.
Root cause: Contaminated sample-compartment optics or a dirty cell holder.
Wavelength accuracy off
MediumSymptoms: Holmium or known-peak check fails.
Root cause: Monochromator wavelength-drive calibration.
Display / printer output fault
LowSymptoms: No printout, dim or garbled display.
Root cause: Display or printer fault.
Serviceable Parts & Consumables
Serviceable items for this model, compiled from its documentation and engineering knowledge for this equipment class. Service class and relative cost are estimates, not manufacturer figures; no part numbers are given. Confirm intervals and pricing against the manufacturer.
Deuterium (UV) lamp
Class Frequent wear itemRel. cost $$$R&R Moderate
Tungsten (visible) lamp
Class Periodic replacementRel. cost $$R&R Moderate
Sample cell holder
Class Major service partRel. cost $R&R Easy
Cuvettes
Class Periodic replacementRel. cost $R&R Easy
| Part | Service class | Relative cost | R&R difficulty |
|---|---|---|---|
| Deuterium (UV) lamp | Frequent wear item | $$$ | Moderate |
| Tungsten (visible) lamp | Periodic replacement | $$ | Moderate |
| Sample cell holder | Major service part | $ | Easy |
| Cuvettes | Periodic replacement | $ | Easy |
Relative cost: $ lowest · $$$$ highest service-part cost for this equipment class. Not a price.
Troubleshooting Approach
A starting sequence for this model, compiled from its documentation and engineering knowledge for this equipment class. Follow the manufacturer's service documentation for the actual procedure.
Symptom: Noisy baseline / low energy
- Run the lamp / performance test.
- Replace the lamp that is low (deuterium for UV noise, tungsten for visible).
- Clean the sample-compartment optics and cell holder.
- Allow the lamps to warm up.
- Re-run the performance and wavelength checks.
Symptom: Wavelength check fails
- Run a holmium or didymium filter scan.
- Compare peak positions to the reference values.
- Recalibrate the wavelength drive per the manual.
- Re-verify.
- Refer for service if it will not hold calibration.
Technical Specs
- Height
- 23 in
- Length
- 14 in
- Weight
- 44 lbs
- Width
- 18 in
Documents & Manuals
Related calculators
From BioLab, for work done with this kind of equipment.
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