Partec CyFlow Space

Partec · Model CyFlow Space

Employing 3 solid state laser light sources. The light source system of the CyFlow® space in its fully equipped configuration comes with a 20mW@488nm blue solid state laser (200mW optional), a 40mW@635nm red diode laser (40mW optional), and a 75mW@405nm violet diode laser or 16mW@375nm UV laser diode, all with direct light coupling for maximum sensitivity. Due to the built-in colour CCD camera, the stability of the sample flow can be visually controlled by a video flow monitor. Featuring unique fluorescence sensitivity. By avoiding fiber coupling, the performance of the CyFlow® instruments is characterized by a superior fluorescence sensitivity of <100 MESF (FITC) and <50 MESF (PE) - see figure belowon the left - and by going to nano-resolution in the scatter channel - see figure below on the right. Any application focussing on small particle detection and analysis, requiring highest fluorescence intensity, fluorescence sensitivity as well as scatter resolution benefits from the unique performancecharateristics of the CyFlow®space. The unique Partec quartz flow cuvetteis the heart of the CyFlow®space, ensuring that particles and cells cross the excitation light with the best possible precision. The design of the flow cuvette incorporates more than 40 years of experience in handling fluids with sub-micrometer and nanoliter precision. Thanks to the optical and mechanical precision of the flow cuvette, superior results are guaranteed for all parameters, e.g. coefficients of variation (CVs) of about 2 % on all fluorescence channels. The sample is transported with the help of a computer controlled digital syringe pump, part of a virtually cross-contamination-free fluid system. Partec Particle and Cell Sorter PPCSas built-in module for safe closed piezo sorting offering highest purity and sorting stability. The Partec particle and cell sorter PPCS is the result of more than 40 years of experience in the field of flow cytometry for research and routine applications in cell and particle characterization. The PPCS can be used as an add-on to the Partec CyFlow®space or the PAS and serves as an optimized module for simplified and precise cell sorting. In order to overcome the problems of droplet sorters (biohazard, contamination) Partec has developed this closed sorting system. The PPCS sorting device can be sterilized safely. Any environmental contamination is completely avoided under normal operation conditions. In addition, the sorting action takes place smoothly. Fragile cells or large particles are sorted without any distortion and other forces acting on the cells during deflection. Long sorting runs (for several hundred milliliters of sample volume) are possible.