Partec CyFlow Space
Partec · Model CyFlow Space
Employing 3 solid state laser light sources. The light source system of the CyFlow® space in its fully equipped configuration comes with a 20mW@488nm blue solid state laser (200mW optional), a 40mW@635nm red diode laser (40mW optional), and a 75mW@405nm violet diode laser or 16mW@375nm UV laser diode, all with direct light coupling for maximum sensitivity. Due to the built-in colour CCD camera, the stability of the sample flow can be visually controlled by a video flow monitor. Featuring unique fluorescence sensitivity. By avoiding fiber coupling, the performance of the CyFlow® instruments is characterized by a superior fluorescence sensitivity of <100 MESF (FITC) and <50 MESF (PE) - see figure belowon the left - and by going to nano-resolution in the scatter channel - see figure below on the right. Any application focussing on small particle detection and analysis, requiring highest fluorescence intensity, fluorescence sensitivity as well as scatter resolution benefits from the unique performancecharateristics of the CyFlow®space. The unique Partec quartz flow cuvetteis the heart of the CyFlow®space, ensuring that particles and cells cross the excitation light with the best possible precision. The design of the flow cuvette incorporates more than 40 years of experience in handling fluids with sub-micrometer and nanoliter precision. Thanks to the optical and mechanical precision of the flow cuvette, superior results are guaranteed for all parameters, e.g. coefficients of variation (CVs) of about 2 % on all fluorescence channels. The sample is transported with the help of a computer controlled digital syringe pump, part of a virtually cross-contamination-free fluid system. Partec Particle and Cell Sorter PPCSas built-in module for safe closed piezo sorting offering highest purity and sorting stability. The Partec particle and cell sorter PPCS is the result of more than 40 years of experience in the field of flow cytometry for research and routine applications in cell and particle characterization. The PPCS can be used as an add-on to the Partec CyFlow®space or the PAS and serves as an optimized module for simplified and precise cell sorting. In order to overcome the problems of droplet sorters (biohazard, contamination) Partec has developed this closed sorting system. The PPCS sorting device can be sterilized safely. Any environmental contamination is completely avoided under normal operation conditions. In addition, the sorting action takes place smoothly. Fragile cells or large particles are sorted without any distortion and other forces acting on the cells during deflection. Long sorting runs (for several hundred milliliters of sample volume) are possible.
Key tips
- ·Run QC controls before trusting any repair - an analyzer that passes self-test can still report clinically wrong results.
- ·Rule out a reagent lot change before opening the instrument - it can look exactly like a hardware fault.
- ·Centrifuge rotors have a hard retirement age/cycle limit regardless of visible condition - don't eyeball it.
Typical Failure Modes
Failure patterns typical of this equipment class - general BMET reference, not verified against this exact unit's own service history unless flagged. Severity is a category-level estimate.
Centrifuge imbalance / bearing
HighSymptoms: Vibration, loud noise, stop error.
Root cause: Worn bearing or unbalanced rotor.
Reagent / wash flow fault
MediumSymptoms: Clogs, high blank, QC fail.
Root cause: Pump tube wear, air in line.
Temperature control fault
MediumSymptoms: Incubator out of range.
Root cause: Failed heater/fan or sensor.
Optics / lamp degradation
LowSymptoms: Low signal, wavelength drift.
Root cause: Lamp/LED or monochromator aging.
Serviceable Parts & Consumables
The parts a BMET typically services on this class of equipment. Service class and relative cost are general engineering estimates for the category, not figures from this unit's documentation. Part numbers appear only when pulled from this unit's own service manual. Always confirm intervals and pricing against the manufacturer.
Incubator heater / fan
Class Periodic replacementRel. cost $$R&R Moderate
Sample aspiration probe
Frequent R&R on high-throughput analyzers.
Class Periodic replacementRel. cost $$$R&R Moderate
Peristaltic pump tubing
Consumable - replace proactively.
Class Frequent wear itemRel. cost $R&R Easy
Xenon / LED light source
Class Periodic replacementRel. cost $$$R&R Moderate
| Part | Service class | Relative cost | R&R difficulty |
|---|---|---|---|
| Incubator heater / fan | Periodic replacement | $$ | Moderate |
| Sample aspiration probe Frequent R&R on high-throughput analyzers. | Periodic replacement | $$$ | Moderate |
| Peristaltic pump tubing Consumable - replace proactively. | Frequent wear item | $ | Easy |
| Xenon / LED light source | Periodic replacement | $$$ | Moderate |
Relative cost: $ lowest · $$$$ highest service-part cost for this equipment class. Not a price.
PM Checklist
Preventive-maintenance tasks typical of this equipment class. Perform to the manufacturer's procedure and your facility's policy - this is general reference, not a schedule for this specific unit.
- ·Daily QC controls run and reviewed
- ·Calibration verification per assay
- ·Routine cleaning and decontamination
- ·Scheduled reagent-path and pump-tubing maintenance
Troubleshooting Approach
A structured starting point derived from the failure modes above. Follow the manufacturer's service documentation for the actual procedure.
Symptom: Sample probe / pipette clog
- Confirm the presentation - typically aspiration error, short sample, carryover - and note the exact alarm or message and the conditions when it occurs.
- Check the most likely cause first: clot/crystal obstruction.
- Isolate the affected subsystem using the manufacturer's functional check or self-test, where one is provided.
- If the fault is confirmed and not a field adjustment, service or replace the sample aspiration probe per the manufacturer's service documentation.
- Return the device to service only after the manufacturer's functional and applicable electrical-safety checks pass.
- Record the findings, any parts used, and the verification results in the maintenance history.
Symptom: Reagent / wash flow fault
- Confirm the presentation - typically clogs, high blank, QC fail - and note the exact alarm or message and the conditions when it occurs.
- Check the most likely cause first: pump tube wear, air in line.
- Isolate the affected subsystem using the manufacturer's functional check or self-test, where one is provided.
- If the fault is confirmed and not a field adjustment, service or replace the peristaltic pump tubing per the manufacturer's service documentation.
- Return the device to service only after the manufacturer's functional and applicable electrical-safety checks pass.
- Record the findings, any parts used, and the verification results in the maintenance history.
Typical Location
Where this class of equipment is typically deployed - general reference, not this unit's own placement record.
- Central / core laboratory
- Point-of-care testing stations
- Blood bank
- Microbiology lab
Documents & Manuals
No documents available for this device yet.
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