Beckman Coulter · Model Cytomics FC 500
More Flexibility for Your Cytometry Applications. • The Cytomics FC 500 Series is a sophisticated product family equipped with the most advanced technologies available. • The FC 500 Series delivers flexible testing, powerful software and a user-friendly interface - features that help scientists optimize their overall workflow efficiency while maintaining true cost effectiveness. • Plus, each FC 500 Series system is backed by Beckman Coulter's unsurpassed reputation for quality and service. Precise, Flexible Analysis Every Time. • Designed for powerful performance and unmatched simplicity Conducts 5-color analysis from either single or dual laser excitation • Improves efficiency by enabling scientists to analyze more antibodies per tube than ever before on an automated system Gives investigators the power to expertly manage every aspect of their flow cytometry studies with the revolutionary CXP software Increased Flexibility with Two Lasers and Multiple Fluorochromes. • Equipped with a collinear second laser that maintains a stable alignment with a single beam path and allows greater versatility in antibody and fluorochrome choices. • Provides versatility to use either PC5 or APC as the fluorochrome for FL4 in multicolor immunophenotyping applications. • Eliminates the need for time delay calculations and the challenges associated with spacially-separated beams. • Wavelength independent detectors facilitate application development Simplified Multicolor Analysis with Advanced Digital Compensation (ADC.) • • Beckman Coulter's ADC technology provides superior correction of spectral overlap and more accurate results. With ADC technology, biological samples can be used for automated color compensation, without the limitations of latex beads. • Only Beckman Coulter flow cytometers offer patented Digital Signal Processing (DSP) for excellent linearity, drift-free amplification and color compensation. The FC 500 with ADC also enables investigators to • Perform digital signal processing with 20-bit data in real-time. • Obtain fast, intuitive compensation feedback to set or fine-tune compensation manually with the QuickCOMP feature. • Increase the visualization of low intensity fluorescence populations • To accommodate numerous configurations, the FC 500's user changeable filters provide fluorochrome selection flexibility. Customized Photomultiplier Tubes (PMTs) detect a broad range of wavelength emissions, which can be used for virtually any dye excited by 488 or 635 nm lasers. Five-Color Analysis From A Single Laser Has Never Been Easier. • Only the FC 500 can be used with a variety of fluorochromes to analyze five colors (FITC, PE, ECD, PC5 or APC and PC7) using either one or two lasers. Investigators could also use Beckman Coulter's IOTest multicolor reagents and drop in their choice of additional conjugated antibodies to make their own combinations. • Performs five-dimensional automated phenotyping analysis with Prism, a unique data reduction display allowing analysis of up to 32 individual phenotypes in a single plot. • Extends multicolor analysis to utilize the full visible emission spectra, which further boosts the level of flexibility that can be achieved
Failure patterns typical of this equipment class - general BMET reference, not verified against this exact unit's own service history unless flagged. Severity is a category-level estimate.
Symptoms: Vibration, loud noise, stop error.
Root cause: Worn bearing or unbalanced rotor.
Symptoms: Clogs, high blank, QC fail.
Root cause: Pump tube wear, air in line.
Symptoms: Incubator out of range.
Root cause: Failed heater/fan or sensor.
Symptoms: Low signal, wavelength drift.
Root cause: Lamp/LED or monochromator aging.
The parts a BMET typically services on this class of equipment. Service class and relative cost are general engineering estimates for the category, not figures from this unit's documentation. Part numbers appear only when pulled from this unit's own service manual. Always confirm intervals and pricing against the manufacturer.
Frequent R&R on high-throughput analyzers.
Consumable - replace proactively.
| Part | Service class | Relative cost | R&R difficulty |
|---|---|---|---|
| Incubator heater / fan | Periodic replacement | $$ | Moderate |
| Sample aspiration probe Frequent R&R on high-throughput analyzers. | Periodic replacement | $$$ | Moderate |
| Peristaltic pump tubing Consumable - replace proactively. | Frequent wear item | $ | Easy |
| Xenon / LED light source | Periodic replacement | $$$ | Moderate |
Relative cost: $ lowest · $$$$ highest service-part cost for this equipment class. Not a price.
Preventive-maintenance tasks typical of this equipment class. Perform to the manufacturer's procedure and your facility's policy - this is general reference, not a schedule for this specific unit.
A structured starting point derived from the failure modes above. Follow the manufacturer's service documentation for the actual procedure.
Where this class of equipment is typically deployed - general reference, not this unit's own placement record.
Describe a symptom or error code. Bio answers from this device's own documented error codes, failure modes, parts, and guides.
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