Beckman Coulter Optima L-100K
Beckman Coulter · Model Optima L-100K
Unmatched versatility, reliability and safety. Capable of generating 802,000 x g at speeds up to 100,000 rpm, Beckman Coulter's Optima L-100 K Ultracentrifuge enables you to perform - in less time - more separations, such as high-purity plasmid DNA, sub-cellular particles and virus isolation. This versatile floor model operates with a broad range of superb rotors, including zonal and continuous flow for large-volume separations. The L-100 K offers the reliability of a vacuum-encased induction drive, the simplicity of user-friendly, microprocessor-based control, and environmentally-friendly cooling system that eliminates the need for CFCs and other harmful liquid refrigerants. All this makes the L-100 K the right choice for your laboratory's routine separation needs.
Key tips
- ·Run QC controls before trusting any repair - an analyzer that passes self-test can still report clinically wrong results.
- ·Rule out a reagent lot change before opening the instrument - it can look exactly like a hardware fault.
- ·Centrifuge rotors have a hard retirement age/cycle limit regardless of visible condition - don't eyeball it.
Typical Failure Modes
Compiled for this model from its own documentation and established engineering knowledge for this equipment class - not from a verified service record. Confirm against the manufacturer's service manual.
Will not start / error at start
HighSymptoms: Error code, run will not begin, or aborts.
Root cause: Set speed exceeds the rotor maximum, an imbalanced load, the lid / door not locked, an unapproved rotor, or a drive / control fault.
Will not reach speed / excessive noise or vibration
MediumSymptoms: Slow spin-up, rumble, or vibration.
Root cause: Load imbalance, worn drive / motor bearings, a worn drive coupling, or (older units) worn motor brushes.
Vacuum will not be achieved (ultracentrifuge)
HighSymptoms: Vacuum-fault alarm, long pump-down, run aborts.
Root cause: Vacuum-pump wear (low oil / worn vanes), a chamber / feed-through seal leak, or a valve fault.
Lid / door will not open / lock
MediumSymptoms: Lid-lock error, will not open after a run, or will not lock to start.
Root cause: Lid-lock solenoid / switch fault, or a run still in progress.
Speed / temperature inaccurate
MediumSymptoms: RCF / RPM or chamber temperature does not match the setting.
Root cause: Tacho / speed-sensor or temperature-sensor drift.
Serviceable Parts & Consumables
Serviceable items for this model, compiled from its documentation and engineering knowledge for this equipment class. Service class and relative cost are estimates, not manufacturer figures; no part numbers are given. Confirm intervals and pricing against the manufacturer.
Drive / motor bearings & coupling
Class Major service partRel. cost $$$R&R Difficult
Lid-lock solenoid / switch
Class Major service partRel. cost $$R&R Moderate
Speed / temperature sensor
Class Major service partRel. cost $$R&R Moderate
Vacuum pump / oil & O-ring kit
Class Periodic replacementRel. cost $$$R&R Difficult
Rotor / rotor O-rings
Log rotor use; retire per the manufacturer's rotor-life programme.
Class Major service partRel. cost $$$R&R Easy
| Part | Service class | Relative cost | R&R difficulty |
|---|---|---|---|
| Drive / motor bearings & coupling | Major service part | $$$ | Difficult |
| Lid-lock solenoid / switch | Major service part | $$ | Moderate |
| Speed / temperature sensor | Major service part | $$ | Moderate |
| Vacuum pump / oil & O-ring kit | Periodic replacement | $$$ | Difficult |
| Rotor / rotor O-rings Log rotor use; retire per the manufacturer's rotor-life programme. | Major service part | $$$ | Easy |
Relative cost: $ lowest · $$$$ highest service-part cost for this equipment class. Not a price.
PM Checklist
Preventive-maintenance tasks typical of this equipment class. Perform to the manufacturer's procedure and your facility's policy - this is general reference, not a schedule for this specific unit.
- ·Daily QC controls run and reviewed
- ·Calibration verification per assay
- ·Routine cleaning and decontamination
- ·Scheduled reagent-path and pump-tubing maintenance
Error Codes
Extracted directly from this unit's own service manual.
DOORThe chamber door is made of high-strength structural steel. A solenoid interlock prevents it from being opened during operation. The door can be opened only by using the door handle and only if the power is on and the vacuum is off, with the chamber at atmospheric pressure. Refer to Section 4, for instructions on accessing the chamber to retrieve a sample in case of a power outage.
SPEEDIf the pulse rate, which is dependent on the number of sectors on the disk and the speed of the rotor, exceeds a set limit, the SPEED diagnostic message will light and the rotor will automatically coast to a stop. When the rotor reaches 1000 rpm, the set speed is checked against the overspeed disk. If the overspeed disk indicates a maximum allowable speed that is lower than the set speed, the set
Troubleshooting Approach
A starting sequence for this model, compiled from its documentation and engineering knowledge for this equipment class. Follow the manufacturer's service documentation for the actual procedure.
Symptom: Will not start / error at start
- Confirm the set speed does not exceed the fitted rotor's maximum and the rotor is approved and correctly seated.
- Rebalance the load - opposing positions evenly loaded.
- Confirm the lid / door latches and locks.
- Look the fault code up in the manual and follow the remedy.
- Functional-check after any repair.
Symptom: Vacuum will not be achieved
- Inspect the chamber / feed-through seal for damage.
- Check the vacuum-pump oil and change it / the O-rings per the schedule.
- Run a leak / vacuum-decay test.
- Rebuild or replace the pump if vacuum is still weak with a good seal.
- Confirm the rotor chamber is clean and dry.
Technical Specs
- Height
- 47.5 in
- Length
- 26.5 in
- Weight
- 1025 lbs
- Maximum Speed
- 100000 rpm
- Width
- 37 in
Typical Location
Where this class of equipment is typically deployed - general reference, not this unit's own placement record.
- Central / core laboratory
- Point-of-care testing stations
- Blood bank
- Microbiology lab
Documents & Manuals
Related calculators
From BioLab, for work done with this kind of equipment.
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