Agilent Technologies 5200 Fragment Analyzer System
Agilent Technologies · Model 5200 Fragment Analyzer System
Low- to mid-throughput separation – Analyze 12 samples per run using parallel capillary electrophoresis Unattended walk-away operation – Load up to three 96-sample microplates, no further user interaction is required Optimized for speed or resolution – Choose from three different capillary array lengths for either fast or high-resolution analysis Minimized instrument preparation time – Reduce the time needed for instrument setup with no daily array handling requirements and room-temperature-stable reagents Broad assay portfolio – Analyze samples ranging from gDNA, NGS libraries, and DNA fragments to total RNA, small RNA, and IVT mRNA vaccines all on one platform High-resolution separation – Achieve separation resolution as good as 3 bp from fragments under 300 bp Objective quality assessment – Qualify your samples using quality metrics for RNA (RQN) and genomic DNA (GQN) Seamless switching between applications – Load two different gels into the instrument to use different kits within one system setup Comprehensive compliance services – Qualify instruments by IQ/OQ services for use in GMP environments.
Key tips
- ·Run QC controls before trusting any repair - an analyzer that passes self-test can still report clinically wrong results.
- ·Rule out a reagent lot change before opening the instrument - it can look exactly like a hardware fault.
- ·Centrifuge rotors have a hard retirement age/cycle limit regardless of visible condition - don't eyeball it.
Typical Failure Modes
Compiled for this model from its own documentation and established engineering knowledge for this equipment class - not from a verified service record. Confirm against the manufacturer's service manual.
No or unstable output voltage / current
HighSymptoms: Run will not start, "no load" or "leak" fault, voltage / current will not hold the setpoint.
Root cause: Power-supply output fault, a bad tank / lead connection, buffer-conductivity issue, or a cracked electrode.
Tank / lid / electrode fault
MediumSymptoms: Leaks at the tank, warped lid will not seat, broken platinum electrode wire.
Root cause: Cracked tank / lid or a broken electrode.
Imaging / gel-doc fault (imagers)
MediumSymptoms: Dark or uneven images, transilluminator / LED not working, camera not detected.
Root cause: Transilluminator LEDs / tubes, the camera, or the imaging software.
Temperature / cooling fault (capillary / large-format)
MediumSymptoms: Runs overheat, poor resolution, temperature alarm.
Root cause: Cooling fan / Peltier or a coolant fault.
PC / software fault
LowSymptoms: Runs will not program, images / data will not save or analyse.
Root cause: PC or software / license.
Serviceable Parts & Consumables
Serviceable items for this model, compiled from its documentation and engineering knowledge for this equipment class. Service class and relative cost are estimates, not manufacturer figures; no part numbers are given. Confirm intervals and pricing against the manufacturer.
Power-supply output board
Class Major service partRel. cost $$R&R Moderate
Tank / lid / electrode set
Class Periodic replacementRel. cost $$R&R Fairly easy
Transilluminator LEDs / tubes (imagers)
Class Periodic replacementRel. cost $$R&R Fairly easy
Camera module (imagers)
Class Major service partRel. cost $$$R&R Moderate
Cooling fan / Peltier
Class Periodic replacementRel. cost $$R&R Moderate
| Part | Service class | Relative cost | R&R difficulty |
|---|---|---|---|
| Power-supply output board | Major service part | $$ | Moderate |
| Tank / lid / electrode set | Periodic replacement | $$ | Fairly easy |
| Transilluminator LEDs / tubes (imagers) | Periodic replacement | $$ | Fairly easy |
| Camera module (imagers) | Major service part | $$$ | Moderate |
| Cooling fan / Peltier | Periodic replacement | $$ | Moderate |
Relative cost: $ lowest · $$$$ highest service-part cost for this equipment class. Not a price.
PM Checklist
Preventive-maintenance tasks typical of this equipment class. Perform to the manufacturer's procedure and your facility's policy - this is general reference, not a schedule for this specific unit.
- ·Daily QC controls run and reviewed
- ·Calibration verification per assay
- ·Routine cleaning and decontamination
- ·Scheduled reagent-path and pump-tubing maintenance
Troubleshooting Approach
A starting sequence for this model, compiled from its documentation and engineering knowledge for this equipment class. Follow the manufacturer's service documentation for the actual procedure.
Symptom: No or unstable output
- Confirm the buffer type / concentration and fill level.
- Check the tank leads and electrode connections and inspect for a broken electrode.
- Test the power supply into a known resistor / dummy load.
- Try a different tank to isolate tank vs supply.
- Replace the power-supply output board if it fails the dummy-load test.
Symptom: Gel-doc image or transilluminator fault
- Confirm the transilluminator / epi-LEDs light.
- Check the camera is detected and the lens / filter are correct.
- Clean the imaging surface.
- Replace failed LEDs / tubes.
- Refer the camera module if it is not detected with a good cable.
Typical Location
Where this class of equipment is typically deployed - general reference, not this unit's own placement record.
- Central / core laboratory
- Point-of-care testing stations
- Blood bank
- Microbiology lab
Documents & Manuals
No documents available for this device yet.
Related calculators
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