Nikon Eclipse E600/E400
Nikon · Model Eclipse E600/E400
Outstanding Optical Performance and Versatility. Biological research microscope with Nikon's revolutionary CFI60 infinity optics for dramatically improved basic performance. Ideal for epi-fluorescence and other sophisticated microscopy, the E600 opens up new dimensions in advanced research applications. Optimized for Extended Clinical Operation. In spite of its solidly stable body, the E400 has a compact design, permitting operations in a relaxed, natural posture. The result: less operator fatigue during long observations. CFI60: Revolutionary Optics. Longest working distance with high numerical aperture objectives. Widest magnification range. Longer parallel optical path. Unequaled fluorescence observation capability. Universal-type objectives System Flexibility for Future Upgrades. A broad range of available attachments. CAE analysis for greater rigidity. Multiple point rigid design Next-Generation Ergonomic Design. Single-hand focus/stage control. Low stage design. Low-angle eyepiece tube (25 degrees). Optional eye level riser. Pushbutton filter slider switching (E600). Refocusing stopper and coarse focus tension control. Super-hard stage surface coating. Ergonomic Binocular tube with tilting function and back and forth adjustment. Auto photo preset switch correct photography.
Typical Failure Modes
Compiled for this model from its own documentation and established engineering knowledge for this equipment class - not from a verified service record. Confirm against the manufacturer's service manual.
Illumination fault
MediumSymptoms: Lamp / LED out, flickering, uneven or dim field, colour-temperature shift.
Root cause: Halogen bulb or LED module at end of life, a lamp-power-supply fault, or a bad connection.
Focus mechanism fault
MediumSymptoms: Coarse / fine focus slips, drifts, binds, or the stage will not hold height.
Root cause: Worn focus-drive gears or slipping tension clutch.
Stage / condenser movement fault
LowSymptoms: Mechanical stage or condenser will not move smoothly or hold position.
Root cause: Worn stage rack-and-pinion or a loose condenser mount.
Optical contamination / poor image
MediumSymptoms: Haze, spots or debris in the field of view that rotate with an element.
Root cause: Dirty objectives, eyepieces, condenser or camera port.
Camera / imaging fault (if fitted)
LowSymptoms: No image, poor colour, software will not connect.
Root cause: Camera, its cable, or the imaging software.
Serviceable Parts & Consumables
Serviceable items for this model, compiled from its documentation and engineering knowledge for this equipment class. Service class and relative cost are estimates, not manufacturer figures; no part numbers are given. Confirm intervals and pricing against the manufacturer.
Illumination bulb / LED module
Class Frequent wear itemRel. cost $$R&R Fairly easy
Lamp power supply
Class Major service partRel. cost $$R&R Moderate
Focus-drive gear / clutch kit
Class Major service partRel. cost $$R&R Difficult
Stage rack-and-pinion
Class Major service partRel. cost $$R&R Difficult
Objective / eyepiece (as damaged)
Class Major service partRel. cost $$$R&R Fairly easy
| Part | Service class | Relative cost | R&R difficulty |
|---|---|---|---|
| Illumination bulb / LED module | Frequent wear item | $$ | Fairly easy |
| Lamp power supply | Major service part | $$ | Moderate |
| Focus-drive gear / clutch kit | Major service part | $$ | Difficult |
| Stage rack-and-pinion | Major service part | $$ | Difficult |
| Objective / eyepiece (as damaged) | Major service part | $$$ | Fairly easy |
Relative cost: $ lowest · $$$$ highest service-part cost for this equipment class. Not a price.
Troubleshooting Approach
A starting sequence for this model, compiled from its documentation and engineering knowledge for this equipment class. Follow the manufacturer's service documentation for the actual procedure.
Symptom: Poor / uneven sections (thick-thin, chatter, scoring)
- Fit a fresh blade and set the correct clearance angle.
- Confirm the specimen clamp and the blade clamp are fully tight.
- Check the block is properly faced and cold enough (cryostat).
- Cut a range of thicknesses to check feed consistency.
- Service the micro-feed mechanism if thickness still drifts.
Symptom: Section thickness inconsistent / feed will not advance or retract
- Verify thickness with a set of test blocks / measured ribbons.
- Check for backlash in the feed mechanism.
- On motorised units, check the stepper / clutch and drive.
- Re-lubricate or replace the micro-feed mechanism.
- Confirm consistent thickness after service.
Symptom: Handwheel binds / brake will not hold / motorised sectioning stalls
- Check for debris or dried grease on the ways.
- Feel the handwheel bearings for roughness.
- Adjust or replace the brake.
- On motorised units, check the drive motor / belt.
- Re-lubricate and confirm smooth, safe operation with the brake holding.
Symptom: Cryostat will not reach temperature / heavy frost (cryostat)
- Check the refrigeration and the chamber / specimen-head temperature sensors.
- Confirm the defrost cycle completes.
- Inspect the door seal.
- Compare chamber temperature to an independent reference.
- Escalate a sealed-system refrigeration fault to a certified tech.
Documents & Manuals
Related calculators
From BioLab, for work done with this kind of equipment.
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